Institute of Zoology, Chinese Academy of Sciences

Group of Molecular Ecology and Evolution

About Our Group

 

 Research Interests

            Our group focuses on phylogeography over China and population history and dynamics of pest speciess. Current work in the group involves population genetic and phylogeographic studies of locusts, cotton bollworm, pine moth, scorpion and frogs, using mitochondrial, microsatellite and nuclear polymorphic DNA markers. Many studies have been carried out on the effects of past climatic changes on patterns of (re)colonization and distribution of genetic diversity in Europe, North America and Australia. Very little is known about that in China or East Asia. We aim at obtaining some picture on this important question.

 

          Long distance migration of some agricultural pest insects affects much the effectiveness of integrated pest management strategies. But first after all, is the so-assumed migration significant? Traditional means to address such questions are not always effective. We would like to investigate the frequency and magnitude of long distance migration in some important pest insects in China, such as the migratory locusts, cotton bollworm, pine moth, using genetic markers.

 

←Swarm of the migratory locust Locusta migratoria

(June 2002, Huanghua, China)

 

 

             Molecular ecology, as an integrated research field, is bridging multiple disciplines. It has provided scientists an unprecedented opportunity to address various difficult biological questions, leading to a revolution of macroscopic biology as a whole. This largely relies on advances in theory, techniques and analysing methods. Our laboratory has never ceased the efforts in their exploration along with some more applied research projects. For example, DNA markers are the primary tools needed for molecular ecology studies, we are interested in the modes and patterns of molecular evolution of these marker sequences. Also, complementary to approaches with mitochondrial and microsatellite DNA markers, analyses with single copy nuclear polymorphic DNA (scnp markers) will play an important roles in genetic studies of populations. These different systems reveal different aspects of a complex story at different perceiving depths. However, the employment of scnp markers in intraspecific studies is not straightforward. We’re trying to seek solutions to make analyses using scnp markers easier.

 

 

 Current Research

1. Ecological and evolutionary consequences of Quaternary climatic changes in East Asia;

2. Molecular ecology of pest species (population dynamics, geographical interactions and behavior and ecological adaptation);

3. Molecular evolution of DNA markers;

4. Methods and techniques for retrieving and analysing genetic data from populations.

 

Some of our past work involving various basic issues in molecular ecology research:

1、Nuclear mitochondrial pseudogenes. The existence of mitochondrial pseudogenes in the nuclear genome poses an annoying problem and also provides some unique opportunity for molecular ecology research. [Two papers are listed by ISI Essential Science Indicators as Highly Cited Papers]

2、Universal DNA markers. The availability of universally applicable DNA markers has greatly promoted molecular ecology research. [One paper is listed by ISI Essential Science Indicators as Highly Cited Paper]

3、Molecular evolution of microsatellite DNA. Microsatellite DNA remains to be the most widely applied molecular markers for genetic study of populations. Some oddity exists for these sequences in lepidopteran insects suggesting mechanism of their molecular evolution.

4、Single copy nuclear polymorphic (scnp) DNA markers. Although challenges still exist, recent progress in genomic studies and molecular biology technologies, and increase of statistical power provide a basis for nuclear DNA analyses to be widely applicable. This will significantly increase our ability to address more difficult population genetic questions, and lead the genetic analysis of populations into a new and flourishing era. [One paper is listed by ISI Essential Science Indicators as Highly Cited Paper, and is among the most read papers in Molecular Ecology]

5、Nuclear haplotype determination. A practical obstacle to employ scnp DNA markers is the general problem of haplotype determination due to the common occurrence of heterozygosity in diploid organisms. We developed a “consensus vote (CV)” approach and demonstrated it to be a reliable strategy for effective haplotype determination of nuclear DNA markers. [more]

 

 

 

 

 

 

 

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